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VOLUME 5, NUMBER 2, July 2005

Research Paper

13. A.B. Mandal, C. Deo, A.V. Elangovan, D.P. Singh and H.P. Shrivastava
Response of Laying Naked Neck X Cari-Red (Hitcari) Hens to Varying Levels of Dietary Protein and Amino Acids During First Phase of Egg Production

14. B.K. Mathur and S.R. Siyak
Utilization of Unfertilized and Fertilized Dhaman (Cenchrus ciliaris) Grass by Marwari Sheep in Arid Region

15. V.D. Joshi, S.M. Wankhede and V.H. Kalbande

Effect of Feeding By-pass Protein with Urea Treated Wheat Straw on Performance of Crossbred (Holstein Friesian X Deoni) Calves

16 Simmi Tomar, S.V.S. Verma, S.K. Bhanja and K. Dhama

Effect of Genotype and Nutrition Interaction on Growth and Nutrient Utilization by Naked Neck and Normally Feathered White Leghorn Chicks

17. N. Muthukumar, J. Rama Prasad and Z. Prabhakara Rao
Evaluation of Legume Tree Leaves and Groundnut Haulms in Sheep

18. P.K. Singh, A.S. Bhat, A.M. Ganai, T.K. Sarkar, H.M. Khan and R. Islam

Effect of Substitution of Groundnut Cake with Mustard Cake on the Growth Performance and Nutrients Utilization of Corriedale Lambs

19. K.K. Singh, S.K. Nag, M.R. Garg and A.B. Mojumdar

Mineral Status of Some Commonly Used Feeds and Forages vis-a-vis their Requirements

20. A.K. Samanta, K.K. Singh, M.M. Das, G.H. Pailan, N.C. Verma and Sonu Rai

Influence of Roughage Source on Rumen Metabolites and Microbial Population with Special Reference to Anaerobic Fungus in Cattle

21. A. DAS
Effect of Nevaro (Ficus hookerii) Leaves Supplementation to Mixed Jungle Grass on Feed Intake and Nutrient Utilization in Sikkim Local Goats

22. S. Saijpaul, R.S. Grewal, Ravneet Kaur and P.K. Naik

Evaluation of Some Potential Complete Rations Economic Milk Production in Crossbred Cows

Short Communications


23. P.C. Sakthivel, A.D. Mercy and T.V. Viswanathan
Effect of Dried Cuttle Fish Waste Silage on Growth Performance in Crossbred (Large White Yorkshire x Desi) Pigs

24. G. Vasudevan and T.V. Viswanathan
Effect of Particle Size of Yellow Maize on the Growth Performance of Cross bred
(Large White Yorkshire X Desi) Pigs

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Research Paper
 
Response of Laying Naked Neck X Cari-Red (Hitcari) Hens to Varying Levels of Dietary Protein and Amino Acids During First Phase of Egg Production

A.B. Mandal, C. Deo, A.V. Elangovan, D.P. Singh and H.P. Shrivastava

Division of Nutrition and Feed Technology
Central Avian Research Institute
Izatnagar-243 122, India

(Received April 08, 2003)


ABSTRACT

An experiment was conducted involving 84 Naked neck x CARI-Red hens (Hitcari), suitable for backyard, during 22-34 weeks of age to evaluate optimum dietary protein and limiting amino acid levels for optimum egg production performance. Four dietary treatments, consisted of different levels of protein (T1 18%, T2 16%, T3 14% and T4 12%) and thus amino acids, were formulated. Each dietary treatment was fed to a total of 21 hens of both feather pattern i.e. naked neck (n=10) and normal feathered hens ((n=11) up to 34 weeks of age. Fifteen eggs from each dietary groups laid on first two consecutive days during 26th, 30th and 34th week of experimental feeding were utilized for quality testing in respect of shape index, albumen index, yolk index, Haugh unit, shell weight and shell thickness. Overall hen-day production was 68.4, 64.9, 62.2 and 61.1% in dietary treatments T1, T2, T3 and T4, respectively. The difference however was not significant even though hens laid more eggs with increased level of CP in the diet. Daily feed intake, egg-weight and egg quality did not differ significantly due to dietary treatments. However, feed conversion efficiency was significantly poor in hens fed 12% CP. The study indicated that a dietary level of 14 to 16% CP with lysine 0.68-0.84%, methionine 0.32-0.34%, methionine +cystine 0.62-0.67% and threonine 0.58-0.68% in diet would be optimum for maximum egg production performance during first phase of production. Feather pattern (naked neck or normal plumage) of birds did not influence egg production or feed utilization.

Key words: Protein, Requirement, Layer, Naked neck, Egg quality, Backyard, Poultry.


Research Paper
 
Utilization of Unfertilized and Fertilized Dhaman (Cenchrus ciliaris) Grass by Marwari Sheep in Arid Region

B.K. Mathur and S.R. Siyak

Division of Animal Science and Forage Production
Central Arid Zone Research Institute
Jodhpur-342 003, India

(Received April 04, 2003)

ABSTRACT

Twelve healthy Marwari ram lambs of about six months age and comparative mean body weight (23.67 kg) were divided in to 3 equal groups viz. T1 (control, maintained on grazing), T2 (stallfed on unfertilized Cenchrus ciliaris) and T3 (stallfed on fertilized C. ciliaris). The T1 animals were sent out daily for 4 h grazing on natural (Cenchrus dominated) pasture, whereas T2 and T3 animals were kept in sheep pan in separate enclosures. Daily consumption of feed and water for both T2 and T3 animals was recorded daily. The body weight of all animals was recorded at weekly interval. During the first 5 weeks, the treatment (T2 and T3) group animals were fed the un-chaffed grass but subsequently switched over to chaffed C. ciliaris grass. The dry matter intake (DMI)/100 kg BW of un-chaffed unfertilized and fertilized grass (2.82±0.19 and 2.73±0.15 kg) was lower than chaffed grass (3.04±0.09 and 3.13±0.11 kg). Water intake (l/100 kg body weight/day) was high (6.46±0.22) in T3 as compared to T2 group (5.39±0.22) in VIII week. The over all 91 days% increase in body weight and average daily gain (ADG) for T1, T2 and T3 groups was 13.68, 14.49 and 14.15% and 55.23±3.16, 38.46±4.81 and 46.78±3.08 g, respectively. The feed palatability and DMI was comparable in lambs fed both unfertilized and fertilized C. ciliaris grass, whereas water intake by animal fed on fertilized grass was on higher side. Body weight gain of unfertilized and fertilized grass was comparable, however, control group showed higher gain. There was no significant difference in various blood parameters studied, however, T2 group showed lower plasma urea values. Sheep reared on free grazing showed higher average daily gain in comparison to those reared exclusively under stall feeding with either fertilized or unfertilized C. ciliaris grass.

Key words: Cenchrus ciliaris, Fertilization, Marwari sheep, Arid region.



Research Paper

Effect of Feeding By-pass Protein with Urea Treated Wheat Straw on Performance of Crossbred (Holstein Friesian X Deoni) Calves

V.D. Joshi, S.M. Wankhede and V.H. Kalbande

Department of Animal Nutrition
College of Veterinary & Animal Sciences
Parbhani - 431 402, India

(Received November 10, 2003)

ABSTRACT

An experiment was carried out to assess the effect of feeding concentrate mixtures varying in by-pass protein levels either with 4 per cent urea treated or untreated wheat straw on performance of twelve crossbred (Holstein Friesian x Deoni) calves (13.04±0.47 m; 130.4±2.3 kg) for a period of 90 days. Two iso-caloric and iso-nitrogenous concentrate mixtures varying in RDP and UDP ratio of 65:35 (CM-I) and 55:45 (CM-II) were prepared. The calves in control group (C) were fed CM-I with ad-libitum untreated wheat straw and those in experimental group (T) were fed CM-II with ad libitum urea treated wheat straw. Average daily dry matter intake (kg), including that through roughage (P<0.01) was significantly (P<0.05) higher in group T as compared to group C. The average daily gain (ADG) was significantly higher (P<0.01) in group T (627.8 g) as compared to those in group C (388.2 g), accompanying a superior (P<0.05) feed efficiency (8.52 vs. 11.37 kg DMI/kg gain). The cost of ration per kg gain in body weight for calves in the two groups was Rs. 24.85 and 18. 36 respectively, indicating a net saving of Rs, 6.49 (25.91 per cent) per kg gain in body weight in T over C. The digestibility coefficients of DM, OM, CP, EE, CF and ADF (P<0.01) and NDF (P<0.05) were significantly higher for experimental ration T as compared to control ration C leading to better nutritive value in the former in terms of DCP (10.1 vs 7.1%) and TDN (63.3 vs 52.9%). The blood urea nitrogen concentration estimated at different intervals after feeding were significantly (P<0.01) lower in calves fed experimental ration T as compared to control ration C, at all intervals. Overall results indicated that in crossbred calves an optimum growth can be achieved by feeding 4 per cent urea treated wheat straw as a basal roughage supplemented with a concentrate mixture containing 20 per cent CP and 45 per cent UDP/by pass protein.

Key words : By-pass protein, Urea treated wheat straw, Crossbred calves.


Research Paper
 
Effect of Genotype and Nutrition Interaction on Growth and Nutrient Utilization by Naked Neck and Normally Feathered White Leghorn Chicks

Simmi Tomar, S.V.S. Verma, S.K. Bhanja and K. Dhama

Division of Nutrition and Feed Technology
Central Avian Research Institute
Izatnagar-243 122, India

(Received January 17, 2004)

ABSTRACT

A bioassay was conducted with White Leghorn (WL) and naked neck (Na) chicks from 0 to 8 week of age to examine the effect of genotype and nutrition interaction. Six test diets combining two ME levels (10.88 or 11.71 MJ/kg) with three protein levels (160, 180 or 200 g/kg) were formulated and the performance of chicks evaluated. The naked neck chicks consumed significantly (P<0.05) more feed than the normally feathered. Although the dietary protein levels had no significant (P>0.05) effect on feed intake of birds but the energy level exerted a highly significant effect. No significant difference in body weight gains of the two genotypes during 0-4 wk was evident but the naked neck birds gained significantly (P<0.01) more weight during 5-8 week period than the normally feathered counter parts. The efficiency of feed conversion was significantly (P<0.01) affected by the genotype as well as by dietary protein and energy level during 0-4 wk period only. Both the genotype and protein levels had no significant effect on the digestibility of dietary nutrients by chicks as examined during the 7th wk of feeding period. The normally feathered birds retained significantly (P<0.05) more nitrogen than the naked neck counter parts. Both the genotypes absorbed more dietary fat from high rather than low energy diets. Ca retention was not significantly affected by the genotype but tended to be more on high-energy diets. The dietary protein level (P<0.05) and not the genotype or energy level affected the utilization of dietary P by birds.

Key words: Naked neck chicks, Growth bioassay, Feed conversion efficiency, Nutrient retention.


Research Paper
 
Evaluation of Legume Tree Leaves and Groundnut Haulms in Sheep

N. Muthukumar, J. Rama Prasad and Z. Prabhakara Rao

Department of Animal Nutrition
College of Veterinary Science
Tirupati-517 502, India

(Received February 16, 2004)


ABSTRACT

Gliricidia (Gliricidia maculata), subabul (Leucaena leucocephala) and sesbania (Sesbania grandiflora) leaves and groundnut (Arachis hypogea) haulms, fed as sole roughage, were evaluated in terms of nutrient digestibility, nitrogen utilization and degradation characteristics of DM and CP using permanently cannulated adult rams. Among the tree leaves CP content (25.6%) was highest in sesbania followed by subabul (19.8%), gliricidia (17.0%) and groundnut haulms (11%). NFE content of legume sources ranged from 45.2-53.5%. The results of metabolism trial with adult rams (24.9±2.1 kg) revealed that the digestibility of DM, OM, CP, CF and NFE was significantly (P<0.01) higher upon feeding of sesbania than those fed with other legume resources. The digestibility (%) of NDF, ADF and cellulose were also significantly higher in rams fed sesbania as compared to those fed other legume. Nitrogen retention (g/d) was significantly higher (P<0.01) in rams fed subabul and sesbania than those fed groundnut haulms and gliricidia. Higher effective dry matter degradability (EDDM) and effective protein degradability (EPD) (%) values were observed for sesbania (61.6 and 52.4) as compared to gliricidia (52.2 and 41.4), subabul (51.7 and 40.2) and groundnut haulms (40.8 and 48.3). It was concluded that subabul and sesbania leaves were better among the leguminous resources evaluated.

Key words: Legume, Tree leaves, Groundnut haulms, Sheep


Research Paper

Effect of Substitution of Groundnut Cake with Mustard Cake on the Growth Performance and Nutrients Utilization of Corriedale Lambs

P.K. Singh, A.S. Bhat, A.M. Ganai, T.K. Sarkar, H.M. Khan and R. Islam

Sheep Research Station
Sher-e-Kashmir University of Agricultural Sciences and Technology (K)
Srinagar-190 006, India

(Received March 19, 2004)

ABSTRACT

A study was undertaken to evaluate the effect of replacement of groundnut cake with mustard cake on the performance of growing lambs. Twenty-four pure-bred Corriedale male lambs of 3-4 months of age with an average body weight of 13.62±0.49 kg, were randomly divided into four groups of six each. The dietary treatments consisted of iso-nitrogenous and iso-caloric concentrate mixtures in which groundnut cake (GNC) was replaced at 0, 50, 75 and 100% levels by mustard oil cake (MOC) and designated as G1, M1, M2 and M3 groups, respectively. The lambs were fed weighed quantity of concentrate mixture and ad libitum chopped oat hay for 90 days. Growth performance and dry matter intakes did not differ significantly among the groups. The average daily gain (g) were 76.63, 75.95, 68.67 and 68.89 in G1, M1, M2 and M3 groups, respectively. Cost of feeding (Rs/kg weight gain) reduced with increasing level of mustard cake in the diet. Feed conversion ratio was lowest (9.04) in 50% GNC group. The animals were in positive nitrogen, calcium and phosphorus balance. Significantly (P<0.05) higher nitrogen balance was observed in M1 in comparison to other groups. It is concluded that groundnut cake may completely be replaced by mustard oil cake without affecting the performance of lambs and economizing the cost of feeding.

Key words : Groundnut cake, Mustard cake, Growth, Nutrient utilization, Lambs


Research Paper

Mineral Status of Some Commonly Used Feeds and Forages vis-a-vis their Requirements

K.K. Singh, S.K. Nag, M.R. Garg and A.B. Mojumdar

Plant Animal Relationship Division
Indian Grassland and Fodder Research Institute
Jhansi-284 003, India

(Received July 05, 2004)

ABSTRACT

Mineral (calcium, phosphorus, magnesium, copper, cobalt, iron, manganese, molybdenum and zinc) content of feeds and forages were estimated in the Bundelkhand region of India and compared with the requirements. The average calcium and magnesium content in both leguminous and non-leguminous forages as well as in concentrate ingredients were sufficient to meet the requirement. Nonleguminous forages were moderate to poor source of phosphorus, whereas, leguminous forages and concentrate supplements were good source of phosphorus. Concentrate supplements were rich in copper, but most of the forages were low in copper except sorghum stover, oats, S. scabra, black gram straw, cowpea and berseem. Almost all feeds and forages had adequate levels of cobalt, iron and manganese while most of the forages were deficient in zinc. Most of the forages were low in molybdenum except for concentrates which were high in Mo. From the present study, it is apparent that zinc was inadequate in many feeds and forages. Therefore, this particular element along with phosphorus and copper should be supplemented in the diets of animals.

Key words: Feeds, Forages, Mineral status, Animal requirement

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Research Paper

Influence of Roughage Source on Rumen Metabolites and Microbial Population with Special Reference to Anaerobic Fungus in Cattle

A.K. Samanta, K.K. Singh, M.M. Das, G.H. Pailan, N.C. Verma and Sonu Rai

Plant Animal Relationship Division
Indian Grassland and Fodder Research Institute
Jhansi-284 003, India

(Received July 12, 2004)

ABSTRACT

The present experiment was aimed to ascertain the effect of roughage source on rumen metabolites and microbial population with special emphasis on anaerobic fungus. Three adult fistulated cattle (BW~400 kg) were maintained on three dietary combinations in switch over design viz. T1: ad libitum feeding of natural grass (predominantly Sehima nervosum and Heteropogon contortus) hay, T2: ad libitum feeding of legume (Lablab purpureus) hay and T3: grazing (8 hours/day) on natural pasture in the month of November-December, respectively. In addition, concentrate was offered @ 1% of the body weight in all dietary combinations. Out of three basal roughage diets, obviously the quality of Lablab purpureus hay was better as it contained 12.67% CP and low level of NDF (51.69%). Total volatile fatty acids (TVFA) concentration of rumen liquor varied from 8.53 to 17.66 mmol/dl and reached to the peak levels at 2 h of post feeding in legume based diet. The rumen ammonia nitrogen concentration ranged from 5.64 to 15.09 mg/dl, with the highest concentration recorded at 1 h of post feeding in the legume based diet. The population of rumen bacteria attained peak level at 2 h of post feeding irrespective of dietary combinations. The rumen anaerobic fungal population ranged from 0.98 x 103 to 8.19 x 103 per ml, being highest at 1 h post feeding in all dietary combinations, as was the case with ciliate protozoa population. The anaerobic fungal isolate viz; isolate 5 belonged to Piromyces sp., while the isolate 9 belonged to Anaeromyces sp. Both the isolates were able to secrete the enzymes (endoglucanase and xylanase) for degradation of structural carbohydrates. The metabolic products of anaerobic fungus included lactic acid, acetic acid, etc. The life cycle of polycentric anaerobic fungus comprised of motile zoospore, which gradually developed to matured sporangia through different intermediate stages. It is concluded that the legume based diets ensured better rumen metabolites for growth and multiplication of rumen bacteria, protozoa as well as fungus than grass hay or grazing on natural pasture. The rumen of cattle possessed both monocentric and polycentric fungus capable to secrete endoglucanase and xylanase for utilization of plant structural carbohydrates.

Key words: Rumen metabolites, Anaerobic fungus, Life cycle, Roughage source


Research Paper

Effect of Nevaro (Ficus hookerii) Leaves Supplementation to Mixed Jungle Grass on Feed Intake and Nutrient Utilization in Sikkim Local Goats

A. Das

ICAR Research Complex for NEH Region
Sikkim Centre, Tadong, Gangtok-737 102, India

(Received August 18, 2004)

ABSTRACT

Four Sikkim local does (average body weight 19.1±2.44 kg) were randomly distributed in an experiment based on Latin square design, comprising of 4 animals, 4 treatments and 4 periods of 21 days each of which 14 days was adaptation period and 7 d was collection period. Four dietary treatments were mixed jungle grass fed ad libitum (I), supplemented with 20 (II), 30 (III) or 40 (IV)% nevaro leaves. Consumption of mixed jungle grass was not significantly affected by increasing level of nevaro leaves supplementation. As a result, total dry matter intake (DMI) increased significantly (P<0.05) with increased level of nevaro supplementation. Digestibility of DM (P<0.01), OM (P<0.01), CP (P<0.05), NDF (P<0.01), ADF (P<0.05) and cellulose (P<0.01) increased only up to 20% level of supplementation. Nitrogen intake (P<0.05), outgo in faeces (P<0.01) and urine (P<0.01) increased linearly with increasing level of nevaro supplementation. Since the increase in outgo was lower than the increase in intake, N balance increased linearly (P<0.01) with increasing level of supplementation. The net impact of increased intake and digestion was a linear increase in intake of DOM (P<0.01) and DCP (P<0.01) with increased levels of dietary nevaro leaves. It was concluded that nevaro leaves supplementation up to 40% level increased intake of DCP and DOM in Sikkim local goats fed mixed jungle grass.

Key words: Ficus hookerii, Mixed grass, Goat, Supplementation, Intake, Digestion.


Research Paper

Evaluation of Some Potential Complete Rations Economic Milk Production in Crossbred Cows

S. Saijpaul, R.S. Grewal, Ravneet Kaur and P.K. Naik

Department of Animal Nutrition
Punjab Agricultural University
Ludhiana-141 004, India

(Received September 19, 2004)

ABSTRACT

An experiment was conducted to evaluate some potential complete rations based on locally available feed ingredients and seasonal fodders for nutrient utilization and economic milk production in crossbred lactating cows. Four complete rations (CR1, CR2, CR3 and CR4) were prepared using concentrate mixture, berseem green, berseem hay, wheat straw and urea fermented wheat straw. The roughage: concentrate ratio in CR1, CR2 and CR3 was 50:50 where as it was 40:60 in CR4. Twenty high yielding crossbred cows of the university dairy farm were randomly divided into four groups of five animals in each group on the basis of their body weight and milk yield and were offered the four complete rations randomly for a period of 90 days. The CP of the treatment complete rations i.e. CR2, CR3 and CR4 ranged between 15-16%. The voluntary dry matter intake (DMI) of the animals in all the groups ranged between 15-20 kg/animal/day. The DMI of animals fed CR3 was significantly (P<0.05) higher than the animals fed other complete rations indicating the positive effect of the inclusion of the green fodder without any dry roughage. The digestibilities of DM, OM, CF and NFE were significantly (P< 0.05) higher in CR3 in comparison to CR2 and CR4 but were comparable to the control (CR1). The total milk yield (18.63±0.81 to 20.80±0.95, l/day), FCM yield (18.21±1.15 to 20.99±1.30, l/day), SNF (8.39±0.01 to 8.48±0.03,%) and fat (3.87±0.04 to 4.14±0.13,%) contents were statistically comparable among the groups. The total cost of milk produced per day was also statistically comparable among the groups. The mean value of the profit earned (Rs) / animal/ day in CR3 (152.86±4.87) group was significantly (P<0.05) higher than CR2 (127.87±3.65) and CR4 (135.07±5.06) groups. The complete ration based on mixture of legume and non-legume fodder was more palatable and was better utilized than the complete rations containing either leguminous or non-leguminous fodder.

Key words: Complete ration, Seasonal fodder, Digestibility, Lactation, Economics.


Short Communication

Effect of Dried Cuttle Fish Waste Silage on Growth Performance in Crossbred
(Large White Yorkshire x Desi) Pigs

P.C. Sakthivel, A.D. Mercy and T.V. Viswanathan

Department of Animal Nutrition
College of Veterinary and Animal Sciences
Mannuthy, Thrissur 680 651, India


(Received June 09, 2004
)

ABSTRACT

An investigation was carried out to study the effect of dried cuttle fish waste silage in the rations of growing and finishing pigs on growth performance and economics of gain. Dried cuttle fish waste silage was used to replace dried fish on protein basis at 0, 50 and 100 per cent, in isocaloric and isonitrogenic grower and finisher rations as dietary treatment T1, T2 and T3, respectively. Average daily gain and feed efficiency (kg feed/kg weight gain) of pigs were 370.31±13.59, 353.15±18.50 and 364.90±15.99 g and 4.16±0.16, 4.28±0.15 and 4.26±0.13 for dietary treatments T1, T2 and T3, respectively, and they did not differ significantly between dietary treatments. The cost of feed per kg live weight gain was Rs. 35.15±1.36, 34.40±1.21 and 32.25±1.03 for treatments T1, T2 and T3, respectively. It could be concluded that dried cuttle fish waste silage can be used as an economical substitute for dried fish in the rations of growing and finishing pigs.

Key words: Crossbred pigs, Dried cuttle fish waste silage, Growth performance, Economics

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Short Communication

Effect of Particle Size of Yellow Maize on the Growth Performance of Cross bred
(Large White Yorkshire X Desi) Pigs

G. Vasudevan and T.V. Viswanathan

Department of Animal Nutrition
College of Veterinary and Animal Sciences
Mannuthy, Thrissur, Kerala-680 651, India

(Received May 30, 2003)

ABSTRACT

Twenty four female, weaned crossbred (Large White Yorkshire X Desi) piglings with an average live weight of 14.5 kg were divided uniformly on the basis of age and body weight into three groups (T1, T2 and T3) of eight animals. They were maintained for 153 days on identical diets containing maize with the particle size of 3.0, 1.0 and <0.1mm, respectively, for the three groups. The piglings were fed grower ration until they attained an average body weight of 50 kg and thereafter switched over to the finisher ration till slaughter. Body weight and body measurements were recorded monthly. The average daily gains of 317, 335 and 373 g were recorded for the groups T1, T2 and T3, respectively. The cumulative feed conversion ratios were 5.38, 5.08 and 4.57 for the groups T1, T2 and T3, respectively. Finely ground maize (<0.1 mm) improved average daily gain and feed conversion efficiency by 6 percent each over medium ground maize (1.0 mm) and by 18 percent and 15 percent over coarsely ground maize (3.0 mm), respectively. Cost of feed per kg live weight of animals in the three groups T1, T2 and T3 were Rs. 42.34, 40.49 and 37.11, respectively.

Key words:Crossbred pig, Yellow maize, Particle size, Feed conversion efficiency, Economics.


   
 

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