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VOLUME 7, NUMBER 1, JANUARY 2007


Research Paper

1. N. PANDA AND HARJIT KAUR
Feeding Synthetic Vitamin E or Milk from Vitamin E Supplemented Murrah Buffaloes on Growth, Immunity and Mineral Status of Calves

2. M. SINGH, K. SHARMA, P. SINGH, N. DUTTA AND U.R. MEHRA
Endogenous Excretion of Purine Derivatives, Creatinine and Nitrogen in Crossbred (Bos indicus x Bos taurus) Cattle

3.P. VASANTHA KUMAR, R. RAVI, M.R. PURUSHOTHAMAN AND B. MOHAN

Performance of Broilers Fed Graded Levels of Mango (Mangifera Indica) Seed Kernel During Finisher Phase

4. 
G. SHYAM SUNDER, N.C.S. GOPINATH, S.V. RAMA RAO AND CH. VIJAYA KUMAR

Quality Assessment of Commercial Feed Grade Salts of Trace Minerals for Use in Poultry Feeds

5. M.K. MONDAL, P. BISWAS AND D. MAZUMDAR
Effect of Supplementation of Two Sources of Copper on Plasma Lipid Profile and Mineral Balance of Black Bengal Goats

6. D.H. REKHATE, V.B. MADAVI AND A.P. DHOK

Performance of Goats Fed Arhar (Cajanus cajan) Straw Based Pelleted Complete Ration

7.B. SAHOO AND T.K. WALLI

Effect of Feeding Rumen Undegradable Protein and Supplemental Energy on Growth Performance and Blood Metabolites in Growing Kids

8. d. KIRAN AND U. KRISHNAMOORTHY

Rumen Fermentation Kinetics and Nitrogen Degradability of Commonly Used Ruminant Feedstuffs In Vitro

9. K. LALU, R. BHAR AND A.B. MANDAL
Effect of Dietary Addition of Bromochloromethane on Nutrient Utilization and Nitrogen Balance in Muzaffarnagari Rams

10. VISHAL MUDGAL, A.K. GARG AND R.S. DASS

Effect of Dietary Selenium and Copper Supplementation on Growth and Nutrient  Utilization in Buffalo (Bubalus Babalis) Calves

11. A.A. JORDAO, JR., A.B. TEIXEIRA-OLIVEIRA, O. FREITAS, C. IZUMI AND H. VANNUCCHI

Effect of Vitamin Supplementation on Lipid Peroxidation Caused by Methotrexate in Rats

12. P.K. NAUK, S. SAIJPAUL AND NEELAM RANI

Evaluation of Rumen Protected Fat Prepared by Fusion Method

Short Communications

13.S.K.S. RAGHUVANSI, R. PRASAD, O.H. CHATURVEDI, A.S. MISHRA, M.K. TRIPATHI, A.K. MISRA, B.L. SARASWAT AND R.C. JAKHMOLA

In Sacco DM Degradability of Brassica Campestris (Mustard) Straw and Pennisetum typhoides (bajra) Kadbi Based Complete Feed Blocks in Sheep

14. R. SENTHIL KUMAR AND HARJIT KAUR
Macro Mineral Status and Dietary Cation-Anion Difference Value of Some Indian Feeds and Fodders

15. L. RADHAKRISHNAN, M. MURUGAN AND T. SIVAKUMAR
Biomass Yield, Chemical Composition and Nutritive Value of Desmanthus virgatus (hedge Lucerne) for Sheep

16. VIJAY K. BHARTI, MEENAKSHI GUPTA, D. LALL, T.C. BALAMURUGAN AND S. IMAM
Effect of Boron on Urinary and Faecal Excretion of Minerals in Buffalo Calves Fed High Fluoride Ration

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Research Paper
 
Feeding Synthetic Vitamin E or Milk from Vitamin E Supplemented Murrah Buffaloes on Growth, Immunity and Mineral Status of Calves

N. Panda and Harjit Kaur*

Division of Dairy Cattle Nutrition
National Dairy Research Institute, Karnal-132001, India

(Received August 18, 2005)

 ABSTRACT

Panda, N. and Kaur, H. 2007. Feeding Synthetic Vitamin E or Milk from vitamin E Supplemented Murrah Buffaloes on Growth, Immunity and Mineral Status of Calves. Animal Nutrition and Feed Technology, 7: 1-10.

Twenty four Murrah buffaloes in advanced pregnancy were randomly divided into 4 groups and were supplemented with vitamin E @ 0, 1000, 1500, 2000 IU from 60d pre-partum to 30d of lactation. The dose was reduced in the supplemented groups to 500, 750, 1000 IU from 30 to 60d of lactation. Eighteen calves born from above 4 groups of buffaloes were divided equally into 3 groups and were fed for 60d; T1-milk from unsupplemented buffaloes, T2-pooled milk from vitamin E supplemented buffaloes, T3–milk from unsupplemented buffaloes+synthetic vitamin E (a-tocopheryl acetate) equivalent to that supplied to T2 calves through milk. The blood samples were collected at 0, 3, 5, 7, 15, 30 and 60 d for analysing plasma immunoglobulin (Ig), total antioxidant activity, a-tocopherol and mineral status. Lymphocyte stimulation index (LSI) was measured at day 0, 5, 7, 15, 30 and 60. The vitamin E intake in T1, T2 and T3 varied from 3.5-12.04; 6.2-21.5 and 8.0-25.0 mg/d respectively during the experimental period. The growth rate was higher in T2 calves. Plasma a-tocopherol concentration was also higher in T2. No treatment effect was seen on plasma total antioxidant activity. Plasma Ig level of 11.20, 9.68 and 10.48 mg/ml on the day of birth increased to 28.59, 37.87 and 31.79 mg/ml after 5d of colostrum feeding in T1, T2 and T3, respectively showing an increase in the T2 than in T1 and T3. An increase (P<0.05) in LSI in T2 (2.16-2.34) calves was seen from day 7th upto 30 d of age in comparison to T1 and T3 (1.73-2.23). The incidence of faecal scour was high in both T1 and T3 than T2. Blood zinc and copper status of T2 calves was higher (P<0.05) than T1 and T3 calves from 7th to 45 days of age. Vitamin E through milk was better utilized than synthetic source in the new born calves as envisaged by higher growth and increased immunity.

Keywords: Vitamin E, Immunity, Calves, Buffaloes, Minerals.

*Corresponding author: harjit1955@msn.com


 
Research Paper

Endogenous Excretion of Purine Derivatives, Creatinine and Nitrogen in Crossbred (Bos indicus x Bos taurus) Cattle

M. Singh, K. Sharma*, P. Singh, N. Dutta and U.R. Mehra

Animal Nutrition Division
Indian Veterinary Research Institute, Izatnagar-243 122, India

(Received June 15, 2006)

Abstract

Singh, M., Sharma, K. Singh, P., Dutta N. and Mehra, U.R. 2007. Endogenous excretion of purine derivatives, creatinine and nitrogen in crossbred (Bos indicus x Bos taurus) cattle. Animal Nutrition and Feed Technology, 7: 11-19.

A study was carried out to elucidate the effect of fasting on endogenous urinary excretion of purine derivatives (PD), creatinine and nitrogen in crossbred (Bos indicus x Bos taurus) cattle. Daily excretion rates (µmol/kgW0.75) of allantoin, uric acid and creatinine during fasting averaged 247.5, 49.1 and 746.3, respectively. The proportional contribution of allantoin (0.82-0.83) and uric acid (0.17-0.18) to total PD excretion (296.7) during pre-fasting and fasting period of the trial remained statistically (P>0.05) similar. The excretion rates of PD decreased significantly during fasting relative to pre-fasting period, however average daily urinary creatinine excretion (0.719-0.746 mmol/kg W0.75/d) remained insignificant irrespective of level of feed offered to animals during various phases of fasting trial. Endogenous urinary nitrogen excretion (g/d or mg/kg W0.75/d) observed during fasting was significantly (P<0.01) lower to the corresponding total N excretion recorded during pre-fasting period. The proportion of allantoin nitrogen to total nitrogen excretion decreased from 14.63 in pre-fasting to 6.57 per cent during fasting period. The creatinine concentration in plasma increased significantly during fasting (P<0.05). Similarly, the glomerular filtration rate (GFR, L/d or L/kg W0.75/d) as proportion of plasma concentration of creatinine (mmol/L) to urinary creatinine excretion (mmol/d) was higher during pre-fasting compared to fasting period. Renal clearance expressed in terms of tubular load of allantoin, uric acid and total PD (mmol/d) showed a significantly (P<0.05) higher rate of tubular load during pre-fasting relative to fasting. Re-absorption of PD (mmol/d or % tubular load) during pre-fasting and fasting followed similar trend as observed for tubular load of its various constituents. The endogenous PD value (297µmol/kg W0.75) obtained during the trial has been used for developing a model for predicting rumen microbial protein supply, however, further studies are warranted to improve the sensitivity and precision for estimating the contribution of endogenous PD in urinary PD excretion by Indian cattle.

Keywords: Crossbred cattle, Endogenous purine derivatives, Creatinine, Nitrogen, Glomerular filtration rate.

*Corresponding Author: hdan@ivri.up.nic.in

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Research Paper

 
Performance of Broilers Fed Graded Levels of Mango (Mangifera Indica) Seed Kernel During Finisher Phase

P. Vasantha Kumar*, R. Ravi, M.R. Purushothaman and B. Mohan

Department of Animal Nutrition,

Veterinary College and Research Institute
Tamil Nadu Veterinary and Animal Sciences University,

Namakkal-637 001, India

(Received October 16, 2004)

Abstract

Kumar, P.V., Ravi, R. Purushothaman, M.R. and Mohan, B. 2007. Performance of broilers fed graded levels of mango (Mangifera indica) seed kernel during finisher phase. Animal Nutrition and Feed Technology, 7: 21-28.

To find out the optimum level of inclusion of mango seed kernel (MSK) in the ration of broiler finishers (4-6 weeks of age), day old broiler chicks (n=144) were assigned to six dietary treatment groups having 12 birds in each group and fed standard broiler starter ration upto 3 weeks of age. The experimental diets (isocaloric and isonitrogenous) containing MSK at 0, 5, 10, 15, 20 and 25 per cent levels replacing maize was fed from 4 to 6 weeks of age. Weight gain was similar to control when MSK was used in the diet upto 20 per cent replacing maize and significantly lowered (P<0.01) in 25 per cent MSK fed group. The retention of nutrients (crude protein, ether extract, crude fibre, calcium and phosphorus) was low (P<0.01) in broilers fed diets containing higher (15% or more) levels of MSK. The feed cost per kg of weight gain increased linearly as the level of MSK increased in the diet. The blood parameters (serum proteins, uric acid, glucose & cholesterol), carcass characteristics (ready-to-cook yield, weights of liver, giblet, trimmable fat & skin) and organoleptic evaluation of meat did not show any significant variation among treatment groups. It can be inferred that inclusion of MSK upto 21 per cent level in the finisher ration per cent did not affect the performance of broiler finishers.

Keywords: Mango seed kernel, Broiler, Feeding value.

*Corresponding Author: drpvkumar@yahoo.com


 
Research Paper
 

Quality Assessment of Commercial Feed Grade Salts of Trace Minerals for Use in Poultry Feeds

G. Shyam Sunder*, N.C.S. Gopinath, S.V. Rama Rao and Ch. Vijaya Kumar

Project Directorate on Poultry
Rajendranagar, Hyderabad-500 030, India

(Received July 08, 2004)

ABSTRACT

Sunder, G.S., Gopinath, N.C.S., Rama Rao, S.V. and Kumar, Ch.V. 2007. Quality assessment of commercial feed grade salts of trace minerals for use in poultry feeds. Animal Nutrition and Feed Technology, 7: 29-35.

Feed grade salts of Mn, Zn, Cu and Fe procured from 6-8 commercial agencies of Hyderabad market were analysed for their elemental content along with the reagent grade salts, using Atomic Absorption Spectrophotometer (AA 100-Perkin Elmer). The salts were primarily in the sulfate form. Zn was available in sulfate and oxide forms. Salts of MnSO4. H2O procured from 7 commercial sources varied significantly (PŁ0.05) in Mn content (1.29-29.15%), but 5 out of 7 salts had a moderate range of elemental content (20.54-29.15%), even if it was significantly lower than the standard salt (31.85%). The variability of Zn content (20.97-30.76%) in ZnSO4.7H2O salts was also statistically significant (PŁ0.05), but 4 out of 6 salts had higher Zn than the standard salt (22.62%). In contrast, only one salt of ZnO had higher Zn (81.98%) than the reagent grade salt (79.94%), indicating wide divergence in the elemental content among the salts. The extent of variability in the commercial salts of CuSO4.5H2O (19.77-28.15%) and FeSO4. 7H2O (14.49-24.24%) was moderate compared to the salts of Mn and Zn. However, significant differences (PŁ0.05) were noticed among the commercial sources of both salts and also, with their respective reagent grade salts. The wide variability in the elemental content of Mn, Zn, Cu and Fe in feed grade salts warranted quantitative estimation of the minerals prior to utilisation in feeds and premixes.

Keywords: Feed grade salts, Sulfates of Mn, Zn, Cu and Fe, Zinc oxide.

*Corresponding Author: pdpoult@ap.nic.inssgajula@rediffmail.com


 
Research Paper

Effect of Supplementation of Two Sources of Copper on Plasma Lipid Profile and Mineral Balance of Black Bengal Goats

M.K. Mondal, P. Biswas* and D. Mazumdar

Department of Animal Nutrition
West Bengal University of Animal and Fishery Sciences
Kolkata-700 037, India

(Received November 16, 2004)

ABSTRACT

Mondal, M.K., Biswas, P. and Mazumdar, D. 2007. Effect of supplementation of two sources of copper on plasma lipid profile and mineral balance of Black Bengal goats. Animal Nutrition and Feed Technology, 7: 37-46.

Twelve healthy castrated 3-4 month old male Black Bengal kids were selected and randomly divided into three groups to study the effect of supplementation of different forms of Cu (CuSO4 and Cu-proteinate) at 10 ppm level on plasma lipid profile and mineral balance. A basal diet (5.7 ppm Cu) was formulated to meet all the nutrient requirements except Cu. Group 1 (C) was offered the basal diet alone, whereas it was supplemented with 10 ppm CuSO4 (T1) and 10 ppm Cu-proteinate (T2) in the treatment groups. A metabolic trial of 5 d duration was conducted after 90 d feeding period. Blood samples were collected at 0 day and subsequently at monthly intervals for studying the plasma lipid profile (cholesterol, high density lipoprotein, total lipid and phospholipids) and mineral profiles (Ca, P, Cu, Fe, Zn and Mn). At 0 d, concentrations of all the plasma lipid profile were similar (P>0.05) among the treatments. In both the Cu supplemented groups, plasma cholesterol was decreased (P<0.05) and high density lipoprotein (HDL), total lipid and phospholipids were increased (P<0.05) as compared to control. Linear effect (P<0.01) of period was found for the entire plasma lipid profile whereas, quadratic effect (P<0.01) was found in plasma HDL and total lipid concentration. Plasma Ca, P, Fe, Zn and Mn were unaffected (P>0.05) by the dietary treatments during the study. From 30 d onwards, the addition of Cu consistently increased (P<0.05) for kids fed Cu from either CuSO4 or Cu-proteinate than control. Urinary excretion of Cu was more (P<0.05) in Cu supplemented kids compared to control. Absorption and retention (%) of Ca, Fe and Zn follow the same trend (P<0.05) as observed in case of Cu. Results inferred that supplementation of Cu in the diet of kids can alter plasma lipid profile and minerals concentration. Further, it can also improve the absorption percentages and retention of minerals. Cu-proteinate was found to be more effective for mineral balance than CuSO4.

Keywords: Plasma lipid, Copper, Kids, Copper-proteinate, Mineral balance.

*Corresponding Author: maicrp@yahoo.com


Research Paper

Performance of Goats Fed Arhar (Cajanus cajan) Straw Based Pelleted Complete Ration

D.H. Rekhate, V.B. Madavi and A.P. Dhok

Department of Animal Nutrition
Post Graduate Institute of Veterinary and Animal Sciences
Akola-444 104, India

(Received January 11, 2005)

ABSTRACT

Rekhate, D.H., Madavi, V.V. and Dhok, A.P. 2007. Performance of goats fed arhar (Cajanus cajan) straw based pelleted complete ration. Animal Nutrition and Feed Technology, 7: 47-52.

An experiment was planned for 110 days to utilize arhar straw based pelleted complete feed in local goats. Twelve non-descript local goats randomly divided into (T1) with average age 10.66±0.70 m and weight 20.83±0.86 and offered sole arhar straw pellets with supplementation of 300 g concentrate pellets 18% CP and 70% TDN and (T2) with average age 10.55±0.17 m and weight 22.40±1.19 kg were fed on arhar straw based pelleted complete diet of 15% CP, 60% TDN. The DMI and DMI/100 kg BW were significantly (P<0.01) more in T1 group. The ADG was 94.83±18.85 (T1) and 83.52±3.43 (T2) g and the feed conversion efficiency was better in T2 group. The digestibility coefficients for different nutrients revealed non significant differences except for NFE (P<0.01). The DCP and TDN of the diets were 8.99±0.39, 58.05±1.65 and 9.52±0.03, 53.41±1.39. All the goats under treatments were in positive nitrogen, calcium and phosphorus balance. The rumen liquor profile study revealed significant (P<0.01) variations for pH, NH3-N, TCA-ppt-N, TN and NPN. It was concluded that both the rations performed well in terms of weight gain and nutrient utilisation and can be fed to the goats for optimum weight gain under intensive system of management.

Keywords: Arhar straw, Nutrient utilisation, Complete feed pellets, Goats.


Research Paper

Effect of Feeding Rumen Undegradable Protein and Supplemental Energy on Growth performance and Blood Metabolites in Growing Kids

B. Sahoo* and T.K. Walli

National Dairy Research Institute
Karnal-132 001, India

(Received August 09, 2005)

ABSTRACT

Sahoo, B. and Walli, T.K. 2007. Effect of feeding rumen undegradable protein and supplemental energy on growth performance and blood metabolites in growing kids. Animal Nutrition and Feed Technology, 7: 53-61.

In an experiment of 120 days duration, twenty healthy growing male kids (Sannen x Beetal and Alpine x Beetal) of 2-3 months of age, weighing 12±0.73 kg were divided into 4 equal groups (TI to T4) in a randomized block design. Two types of isonitrogenous and isocaloric concentrate mixtures (CM-I and CM-II) were fed to group T1, T2, T3 and T4, respectively The two concentrate mixtures were similar in composition except RDP:UDP ratio (73:27 and 59:41). Animals in T2 and T4 were additionally supplemented with molasses at the rate (8% CM on DM basis). All the animals were fed roughages (Berseem:wheat straw i.e. 2:1) to meet 50 per cent of DM intake. Blood samples were collected at 40, 80 and 120 days of post feeding and plasma samples were analysed. Plasma albumin and a-AAN levels were highest (P<0.05) in group T4, fed high UDP along with energy. However, plasma urea concentration was lower (P<0.05) in group T3 and T4 than T1 at 80 and 120 days of post feeding. DM intake in control group was lower (P<0.01) than to other 3 groups. Average daily gain (ADG) recorded was 47.6, 52.3, 62.9 and 71.4 in groups T1, T2, T3 and T4, respectively, with the values being significantly higher (P<0.05) in T4 than in group T1, but at par with T2 and T3. Feed conversion efficiency was also significantly higher (P<0.05) in group T4 (10.14) than T1 (7.28) and T2 (7.47) but similar with T3 (9.35). It is thus inferred that additional supply of energy in the form of molasses along with undegradable protein positively influenced the growth rate and feed conversion efficiency without any significant effect on blood metabolites.

Keywords: Mustard cake, Undegradable protein, Molasses, Blood metabolites, Kids.

*Corresponding Author: biswanath_72@yahoo.co.in

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Research Paper

Rumen Fermentation Kinetics and Nitrogen Degradability of Commonly Used Ruminant Feedstuffs In vitro

D. Kiran* and U. Krishnamoorthy

Department of Livestock production and Management
Karnataka Veterinary, Animal and Fisheries Sciences University
Veterinary College, Hebbal-560 024, India

(Received March 24, 2005)

ABSTRACT

Kiran, D. and Krishnamoorthy, U. 2007. Rumen fermentation kinetics and nitrogen degradability of commonly used ruminant feedstuffs in vitro. Animal Nutrition and Feed Technology, 7: 63-71.

The study was conducted to evaluate tropical feedstuffs used in feeding ruminants for rumen fermentation kinetics and N fractions. The feed samples selected for the study included 8 protein sources, 9 energy sources, 8 unconventional byproducts, 5 roughages and 6 complete diets. The D (potential gas production, ml/g DM), and k (h-1) (rate of gas production) for protein sources varied from 141.0 to 296.5 and 0.0858 to 0.1562, for energy sources from 327.0 to 476.5 and 0.0544 to 0.1078, for unconventional byproducts from 46.3 to 495.9 and 0.0233 to 0.1117, for roughages from 245.9 to 303.8 and 0.0219 to 0.1092 and for the six diets it varied from 218.4 to 301.8 and 0.0553 to 0.0640. Total N content (g/kg DM, Mean±SD) for protein sources, energy sources, unconventional byproducts, roughages and complete diets was 54.4±14.6, 15.8±7.2, 11.1±6.7, 16.7±9.6 and 20.2±0.13, respectively. Potentially digestible N was more than 90 per cent for most of the feedstuffs with the exception of tapioca peel (82.3%), tapioca meal (76.4%), coarse wheat bran (83.9%), Bengal gram husk (82.9%), chicory bran powder (83.3%), poha bran (75.0%), ragi husk (78.1%), finger millet straw (71.1%), hybrid napier (76.1%) and rice straw (85.8%). However, among the protein sources, the N rapidly degraded in the rumen was maximum in ground nut meal (59.7%), followed by rapeseed meal (35.5%), gingelley cake (32.9%), ambadi cake (31.5%), cotton seed meal (24.4%), coconut meal (20.1%), soybean meal (17.2%) and sunflower meal (6.5%). The N solubility in buffer and detergent solutions and fermentation kinetics within one class of feedstuffs may be an indicator of variability in the degradation of protein and energy, respectively in rumen.

Keywords: Nitrogen solubility, Digestible nitrogen, Fermentation characteristics.

*Corresponding Author: kirandoranalli@yahoo.com


Research Paper

Effect of Dietary Addition of Bromochloromethane on Nutrient Utilization and Nitrogen Balance in Muzaffarnagari Rams

K. Lalu*, R. Bhar and A.B. Mandal

Centre for Advanced Studies in Animal Nutrition
Indian Veterinary Research Institute, Izatnagar-243 122, India

(Received June 04, 2006)

ABSTRACT

Lalu, K., Bhar, R. and Mandal, A.B. 2007. Effect of dietary addition of bromochloromethane on nutrient utilization and nitrogen balance in Muzaffarnagari rams. Animal Nutrition and Feed Technology, 7: 73-78.

The present study was conducted to assess the effect of bromochloromethane (BCM), a potent antimethanogenic compound, as feed additive on intake, nutrient utilization, and nitrogen balance in rams fed on diet containing maintenance (NP1; NRC, 1985) and sub-maintenance level (NP2; 20% less DE of NRC, 1985) of energy (NRC, 1985) with adequate protein. Sixteen adult Muzaffarnagari rams of comparable bodyweight (40.25±0.65 kg) were divided into four equal groups, were fed these two diets supplemented with 0.04ml BCM (BCM1) or without BCM (BCM0) capsules. Results from the 4 experimental diets (NP1BCM0, NP1BCM1, NP2BCM0 and NP2BCM1) were tested using 2 X 2 factorial designs. The overall daily feed intake in respective groups were 817.9±19.43, 763.1±10.42, 568.7±15.78 and 592.6±15.78 g/d, the values being numerically lower in NP2BCM0 and NP2BCM1 than that of NP1BCM0 and NP1BCM1. Digestibility of nutrients did not differ significantly among the different interacting groups and also between NP1 and NP2 or between BCM0 and BCM1. Non significant difference was also observed between the values of N balances in rams among the different interacting groups as well as between NP1 and NP2, and BCM0 and BCM1. Results of the present study revealed that BCM, a potent antimethanogenic compound could be added in the diet of rams without affecting performance.

Keywords: Bromochloromethane, Nutrient utilization, Nitrogen balance, Rams.

*Corresponding Author: lalukv1@rediffmail.com


 

Research Paper

Effect of Dietary Selenium and Copper Supplementation on Growth and Nutrient Utilization in Buffalo (Bubalus Bubalis) Calves

 

Vishal Mudgal, A.K. Garg* and R.S. Dass

Center of Advanced Studies in Animal Nutrition
Indian Veterinary Research Institute, Izatnagar-243 122, India

(Received July 24, 2006)

ABSTRACT

Mudgal, V., Garg, A.K. and Dass, R.K. 2007. Effect of dietary selenium and copper supplementation on growth and nutrient utilization in buffalo (Bubalus bubalis) calves. Animal Nutrition and Feed Technology, 7: 79-88.

Twenty male buffalo (Bubalus bubalis) calves of 8-9 months of age (112.1±7.69kg mean body weight) were divided into four treatment groups (n= 5 per group). Animals in the control (T1) group were fed a standard diet comprising of concentrate mixture (20% maize, 27% soybean meal, 50% wheat bran, 2% mineral mixture and 1% common salt) and ad libitum wheat straw. Animals in the experimental groups were also fed same basal diet except that they were supplemented either with 0.3ppm selenium (Se) (T2), 10ppm of copper (Cu) (T3) or both 0.3ppm Se and 10ppm Cu (T4). Experimental feeding lasted for 120 days including a 6 days metabolism trial. The intake of DM and OM, and digestibility of DM, OM, CP, EE, NDF and hemicellulose were similar (P>0.05) among the four groups. However, digestibility of ADF and cellulose was significantly higher in the T2 group. While supplementation of Se/Cu had no effect on N, Ca and P balance, feed: gain ratio and DCP intake, TDN intake was significantly (P<0.05) higher in group T2 and T3. The growth rate (g/d) of the buffalo calves was also significantly (P<0.05) increased in group T3 (465) and T4 (447) as compared to the control T1 (406). These results suggested that Se and Cu requirements of buffaloes might be higher than the presently recommended levels of NRC for cattle.

Keywords: Buffalo calves, Selenium, Copper, Growth, Nutrient utilization.

*Corresponding Author: garg@ivri.up.nic.in


 

Research Paper

Effect of Vitamin Supplementation on Lipid Peroxidation Caused by Methotrexate in Rats

A.A. Jordao*, Jr., A.B. Teixeira-Oliveira, O. Freitas, C. Izumi and H. Vannucchi

Nutrition Division, Department of Internal Medicine
Faculty of Medicine of Ribeirao Preto-USP, Brazil

(Received September 07, 2006)

ABSTRACT

Jordao, A.A Jr., Teixeira-Oliveira, A.B., Freitas, O., Izumi, C. and Vannucchi, H. 2007. Effect of vitamin supplementation on lipid peroxidation caused by methotrexate in rats. Animal Nutrition and Feed Technology, 7: 89-94.

The methotrexate (MTX) is an antitumoral agent, acting like an antagonist of the folic acid and the administration of the MTX increases free radical production and induces hepatorenal oxidative injury in rats. The aim of the present study was to assess the value of adding a high dose of vitamin mixture to rats in lipid peroxidation provoked by methotrexate, and the role of vitamin E against the free radical production. The study utilized 40 rats subdivided in 4 groups: Group A received a diet with 3 times the recommendation of vitamin mixture according to American Institute of Nutrition diets for rodents, published in 1993 (AIN93); Group B, the same diet with normal, one time, vitamin mixture according to AIN-93; rats in Group C received a diet with 3 times vitamin mixture according to American Institute of Nutrition diets for rodents, published in 1976 (AIN76); Group D, the same diet with normal, one time, vitamin mixture according to AIN76. The increase in hepatic vitamin E and glutathione and decrease of lipid peroxidation levels with suplemmentation of 3 times vitamin mixture can be able to protect for the possible adverse effects of the MTX administration in the oxidative stress in rats.

Keywords: Methotrexate, Lipid Peroxidation, Vitamin E, Diet, Antioxidants, Glutathione, Rats.

*Corresponding Author: alceu@fmrp.usp.br


 

Research Paper

 

Evaluation of Rumen Protected Fat Prepared by Fusion Method

P.K. Naik*, S. Saijpaul and Neelam Rani

Department of Animal Nutrition
Guru Angad Dev Veterinary and Animal Sciences University
Ludhiana-141 004, India

(Received October 23, 2006)

ABSTRACT

Naik, P.K., Saijpaul, S. and Rani, N. 2007. Evaluation of rumen protected fat prepared by fusion method. Animal Nutrition and Feed Technology, 7: 95-101.

An experiment was chalked out to prepare rumen protected fat viz. calcium salts of long chain fatty acids (LCFA-Ca) by fusion method from palm fatty acid oil (PO), rice bran fatty acid oil (RBO) and mixed fatty oil (MO). The fat% of the PO (97.80) and RBO (96.46) were similar. The iodine number of RBO (75.18) was higher than the PO (49.67). Twenty types of rumen protected fat each of PO and RBO were prepared by treating with technical grade calcium hydroxide under specific conditions viz. with and without sulphuric acid, with and without steam and at different treatment times (5, 10, 20, 25 and 30; minutes). Studies indicated that use of acid was beneficial to obtain more FFAs from the oils for higher yield of rumen protected fat, steam had no impact and 30 minutes treatment time was optimum for the preparation of rumen protected fat. The percent mean values of yield, fat and total ash of the RBO based with acid and without steam at 30 minutes treatment time rumen protected fat was 78.09, 76.00 and 15.58, respectively, and was statistically similar with the respective mean values of all other types of rumen protected fat. There was no significant difference in the Ca% and DM, OM and fat undegradability values of the different types of rumen protected fat and were ranged from 4.93 to 5.88; 84.30 to 89.02; 82.88 to 89.95 and 84.23 to 87.49, respectively. For the preparation of rumen protected fat for practical purpose, 30 minutes treatment time, use of sulphuric acid, without steam should be preferred. Besides, between PO and RBO, the RBO was preferred due to its higher degree of unsaturation of fatty acids.

Keywords: Rumen, Protected fat, Calcium salts, Long chain fatty acids, Fusion method.

*Corresponding Author: pknaikpau@rediffmail.com


 

Short Communication

 
In Sacco DM Degradability of Brassica Campestris (mustard) Straw and Pennisetum typhoides (bajra) Kadbi Based Complete Feed Blocks in Sheep

S.K.S. Raghuvansi, R. Prasad, O.H. Chaturvedi, A.S. Mishra,

M.K. Tripathi*, A.K. Misra, B.L. Saraswat and R.C. Jakhmola

Division of Animal Nutrition, Central Sheep and Wool Research Institute
Avikanagar-304 501, India

(Received March 02, 2005)

ABSTRACT

Raghuvansi, S.K.S., Prasad, R., Chaturvedi, O.H., Mishra, A.S., Tripathi, M.K., Misra, A.K., Saraswat, B.L. and Jakhmola, R.C. 2007. In sacco DM degradability of Brassica campestris (mustard) straw and Pennisetum typhoides (bajra) kadbi based complete feed blocks in sheep. Animal Nutrition and Feed Technology, 7: 103-109.

A total of eight test diets were prepared by compressing the feed mixture at 4000 psi using a horizontal feed block-making machine. Concentrate mixture (25 parts) and molasses (5 parts) were added in each treatment. Treatments were comprised of mustard straw (MS 70 parts; T1), MS plus dried neem (Azadirachta indica) leaves (40:30 parts; T2), MS plus dried siris (Albizia lebbeck) leaves (40:30 parts; T3) and MS plus dried ardu (Ailanthus excelsa) leaves (40:30 parts; T4). Similarly, in treatments T5, T6, T7 and T8 the bajra kadbi was used as roughage in place of mustard straw. The incorporation of tree leaves in mustard straw based complete feed significantly (P<0.05) increased the instantly degradable fraction (a), rate of degradation (c) and the potential degradation (a+b) of the test diets. The coefficients of effective degradability (ED) at a rumen out flow rate of 0.05 per hour were also higher (P<0.05) in the treatment combinations where tree leaves replaced the 30 parts of mustard straw in the diet. However, the slowly or potentially degradable fraction of the diet (b) remains unaffected due to addition of tree leaves in the MS based diets. Among the tree leaves, the ardu leaves were found most effective in improving the ruminal degradation of the mustard straw based diets. The ruminal degradability coefficients for bajra kadbi based diets were higher than that of mustard straw based diets. Incorporation of tree leaves in bajra kadbi based diets did not improve the degradation kinetics, rather significantly (P<0.05) reduced the instantly and slowly degradable fractions of the diet. It is, therefore, concluded that incorporation of tree leaves in mustard straw based complete feed blocks improved its ruminal DM degradation. However, the incorporation of tree leaves in bajra kadbi based diets did not show any appreciable improvement in DM degradation.

Keywords: Mustard straw, Bajra kadbi, Complete feed block, In sacco degradation.

*Corresponding Author: mktripathi@gmail.com


Short Communication

Macro Mineral Status and Dietary Cation-Anion Difference Value of Some Indian Feeds and Fodders

R. Senthil Kumar and Harjit Kaur*

Dairy Cattle Nutrition Division
National Dairy Research Institute, Karnal-132 001, India

(Received February 11, 2005)

ABSTRACT

Kumar, R.S. and Kaur, H. 2007. Macro mineral status and dietary cation-anion difference value of some Indian feeds and fodders.Animal Nutrition and Feed Technology, 7: 111-117.

Calcium, phosphorus, sodium, chlorine, potassium, magnesium and sulphur contents of commonly used feeds and fodders were estimated for calculating their dietary cation anion difference (DCAD) value. Calcium and phosphorus contents among different concentrate ingredients varied from (0.11-0.67%) and (0.28-1.61%) showing more than 1:2 ratio of Ca to P. Calcium concentration in forages was much higher than in cereal grains. Maize and mustard fodder showed Ca: P ratio of 2:1, whereas berseem contained five times more Ca than phosphorus. Rice bran, wheat bran and oilseed meals were especially rich in P as compared to other feed sources. Mg content in wheat and wheat straw was low (0.15 ppm) as compared to other feedstuffs. There was huge variation in Na (0.27 - 1.30%) and K (0.56-3.36%) content of different feeds. Sulphur content ranged from (0.27-0.92%) with an average value of 0.45 per cent in concentrates and 0.64-0.94 with average value of 0.78 per cent in roughages. Maximum variation was observed in chloride content of different feeds. Five different equations were employed to calculate the DCAD value of different feeds and fodders. Amongst concentrate ingredients, DCAD value of rice bran was lowest (-322.37 meq/100g DM) because of its high chloride content (10.59 %). Among roughages, mustard had lowest DCAD value of -21.86 and berseem had highest DCAD value of 40.79 meq/100g DM.

Keywords: Calcium, Phosphorus, Sodium, Chlorine, Potassium.

*Corresponding Author: harjit1955@msn.com

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Short Communication

Biomass Yield, Chemical Composition and Nutritive Value of Desmanthus virgatus (hedge lucerne) for Sheep
 
L. Radhakrishnan*, M. Murugan and T. Sivakumar
Livestock Research Statione
Kattupakkam-603 203, India

(Received March 09, 2005)

ABSTRACT

Radhakrishnan, L., Murugan, M. and Sivakumar, T. 2007. Biomass yield, chemical composition and nutritive value of Desmanthus virgatus (hedge lucerne) for sheep. Animal Nutrition and Feed Technology, 7: 119-123.

An experiment was conducted to study the biomass yield, palatability, chemical composition and nutritive value of Desmanthus virgatus in sheep. The biomass yield per acre for six cuttings was 39.81 tons and the mean crude protein and crude fibre content were 15.20 and 19.77 percent. The mean dry matter intake in terms of percent body weight and metabolic body weight was 4.97 percent and 104.52 g per kg. The DCP and TDN content were 10.86 and 59.79 percent. From the results obtained in the present study, it is apparent that Desmanthus virgatus fodder can be used as potential leguminous fodder source for small ruminants.

Keywords: Desmanthus virgatus, Biomass yield, Chemical composition, Nutritive value, Sheep.

*Corresponding Author: radhakrish67@yahoo.com

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Short Communication

Effect of Boron on Urinary and Faecal Excretion of Minerals in Buffalo Calves Fed High Fluoride Ration

Vijay K. Bharti*, Meenakshi Gupta, D. Lall, T.C. Balamurugan and S. Imam

Department of Veterinary Physiology
Choudhry Charan Singh, Haryana Agricultural University, Hisar-125 004, India

(Received March 23, 2005)

ABSTRACT

Bharti, V.K., Gupta, M., Lall, D., Balamurugan, T.C. and Imam, S. 2007. Effect of boron on Urinary and faecal excretion of minerals in buffalo calves fed high fluoride ration. Animal Nutrition and Feed Technology, 7: 125-130.

In order to assess the ameliorative effect of boron (B) in the buffalo calves fed high fluoride (F) diet, 12 male Murrah buffalo calves of 6-8 month old were divided into three groups. Fluoride was added in the ration (as sodium fluoride, NaF) to make 60 ppm F on DM basis in group II and III were compared to group I (control). Boron was added in the ration (as borax, Na2B4O7.10H2O) to make 140 ppm B on DM basis in group III. After 90 days of experimental feeding, a metabolism trail of 7 days duration was conducted. Urine and faecal samples were collected and analysed for fluorine (F), calcium (Ca), phosphorus (P), iron (Fe), zinc (Zn) and copper (Cu). The level of F (@60 ppm) as well as B in ration did not cause a significant change in P, Zn, Cu and Fe excretion in urine and faeces. However, urinary F (42.29±2.62 ppm) and Ca (0.56±0.01) g/head/day excretion was significantly (P<0.05) higher in group II due to F addition. Further, faecal F excretion was significantly (P<0.05) higher (91.52±4.60 ppm) in group III compared to other groups due to F and B in group III. Results indicated that boron has ameliorative effect on high F intake as it induced removal of F through faeces.

Keywords: Boron, Buffalo, Fluoride, Minerals, Urine.

*Corresponding Author: vijaykbharti@rediffmail.com


   
 

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